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Chromatin accessibility is associated with CRISPR-Cas9 efficiency in the zebrafish (Danio rerio)

CRISPR-Cas9 technology is routinely applied for targeted mutagenesis in model organisms and cell lines. Recent studies indicate that the prokaryotic CRISPR-Cas9 system is affected by eukaryotic chromatin structures. Here, we show that the likelihood of successful mutagenesis correlates with transcri...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Uusi-Mäkelä, Meri I. E., Barker, Harlan R., Bäuerlein, Carina A., Häkkinen, Tomi, Nykter, Matti, Rämet, Mika
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912780/
https://ncbi.nlm.nih.gov/pubmed/29684067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196238
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