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Titratable Avidity Reduction Enhances Affinity Discrimination in Mammalian Cellular Selections of Yeast-Displayed Ligands

Yeast surface display selections against mammalian cell monolayers have proven effective in isolating proteins with novel binding activity. Recent advances in this technique allow for recovery of clones with even micromolar binding affinity. However, no efficient method has been shown for affinity b...

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Detalhes bibliográficos
Publicado no:ACS Comb Sci
Main Authors: Stern, Lawrence A., Csizmar, Clifford M., Woldring, Daniel R., Wagner, Carston R., Hackel, Benjamin J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5521271/
https://ncbi.nlm.nih.gov/pubmed/28322543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acscombsci.6b00191
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