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Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections

Selections of yeast-displayed ligands on mammalian cell monolayers benefit from high target expression and nanomolar affinity, which are not always available. Prior work extending the yeast–protein linker from 40 to 80 amino acids improved yield and enrichment but is hypothesized to be below the opt...

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Bibliografske podrobnosti
izdano v:Protein Eng Des Sel
Main Authors: Lown, Patrick S, Cai, Jessy J, Ritter, Seth C, Otolski, Jacob J, Wong, Ryan, Hackel, Benjamin J
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2021
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC8058008/
https://ncbi.nlm.nih.gov/pubmed/33880560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzab004
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