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Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections
Selections of yeast-displayed ligands on mammalian cell monolayers benefit from high target expression and nanomolar affinity, which are not always available. Prior work extending the yeast–protein linker from 40 to 80 amino acids improved yield and enrichment but is hypothesized to be below the opt...
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| Publié dans: | Protein Eng Des Sel |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8058008/ https://ncbi.nlm.nih.gov/pubmed/33880560 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzab004 |
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