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Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from cell cult...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Falck, David, Haberger, Markus, Plomp, Rosina, Hook, Michaela, Bulau, Patrick, Wuhrer, Manfred, Reusch, Dietmar
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2017
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5509673/
https://ncbi.nlm.nih.gov/pubmed/28706253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-05641-1
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