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Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from cell cult...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Falck, David, Haberger, Markus, Plomp, Rosina, Hook, Michaela, Bulau, Patrick, Wuhrer, Manfred, Reusch, Dietmar
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5509673/
https://ncbi.nlm.nih.gov/pubmed/28706253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-05641-1
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