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Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Erythropoietin (EPO) is a heavily glycosylated hormone whose recombinant forms are used for treatment of anaemia. EPO glycosylation is important for its pharmacological properties. An analytical workflow, which can determine EPO glycosylation in an accurate and high-throughput fashion from cell cult...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Falck, David, Haberger, Markus, Plomp, Rosina, Hook, Michaela, Bulau, Patrick, Wuhrer, Manfred, Reusch, Dietmar
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5509673/
https://ncbi.nlm.nih.gov/pubmed/28706253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-05641-1
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