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Catalytic-site design for inverse heavy-enzyme isotope effects in human purine nucleoside phosphorylase
Heavy-enzyme isotope effects ((15)N-, (13)C-, and (2)H-labeled protein) explore mass-dependent vibrational modes linked to catalysis. Transition path-sampling (TPS) calculations have predicted femtosecond dynamic coupling at the catalytic site of human purine nucleoside phosphorylase (PNP). Coupling...
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| 出版年: | Proc Natl Acad Sci U S A |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
National Academy of Sciences
2017
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5488955/ https://ncbi.nlm.nih.gov/pubmed/28584087 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1704786114 |
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