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Isotope-specific and amino acid-specific heavy atom substitutions alter barrier crossing in human purine nucleoside phosphorylase

Computational chemistry predicts that atomic motions on the femtosecond timescale are coupled to transition-state formation (barrier-crossing) in human purine nucleoside phosphorylase (PNP). The prediction is experimentally supported by slowed catalytic site chemistry in isotopically labeled PNP ((1...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Suarez, Javier, Schramm, Vern L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4568649/
https://ncbi.nlm.nih.gov/pubmed/26305965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1513956112
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