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Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels
Transition path-sampling calculations with several enzymes have indicated that local catalytic site femtosecond motions are linked to transition state barrier crossing. Experimentally, femtosecond motions can be perturbed by labeling the protein with amino acids containing (13)C, (15)N, and nonexcha...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6142206/ https://ncbi.nlm.nih.gov/pubmed/29915028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1805416115 |
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