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Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels
Transition path-sampling calculations with several enzymes have indicated that local catalytic site femtosecond motions are linked to transition state barrier crossing. Experimentally, femtosecond motions can be perturbed by labeling the protein with amino acids containing (13)C, (15)N, and nonexcha...
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| 發表在: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
National Academy of Sciences
2018
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6142206/ https://ncbi.nlm.nih.gov/pubmed/29915028 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1805416115 |
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