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Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels

Transition path-sampling calculations with several enzymes have indicated that local catalytic site femtosecond motions are linked to transition state barrier crossing. Experimentally, femtosecond motions can be perturbed by labeling the protein with amino acids containing (13)C, (15)N, and nonexcha...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Harijan, Rajesh K., Zoi, Ioanna, Antoniou, Dimitri, Schwartz, Steven D., Schramm, Vern L.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2018
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6142206/
https://ncbi.nlm.nih.gov/pubmed/29915028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1805416115
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