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Remote Mutations and Active Site Dynamics Correlate with Catalytic Properties of Purine Nucleoside Phosphorylase

It has been found that with mutation of two surface residues (Lys(22) → Glu and His(104) → Arg) in human purine nucleoside phosphorylase (hPNP), there is an enhancement of catalytic activity in the chemical step. This is true although the mutations are quite remote from the active site, and there ar...

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Detalhes bibliográficos
Main Authors: Saen-Oon, Suwipa, Ghanem, Mahmoud, Schramm, Vern L., Schwartz, Steven D.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2367194/
https://ncbi.nlm.nih.gov/pubmed/18234834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.121913
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