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Construction of a Gene Knockdown System Based on Catalytically Inactive (“Dead”) Cas9 (dCas9) in Staphylococcus aureus

There has been an absence of an efficient method of gene knockdown in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The previously developed antisense RNA technology is mainly applied for forward genetic screening but is rather limited in specific gene knock...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Zhao, Changlong, Shu, Xueqin, Sun, Baolin
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5452804/
https://ncbi.nlm.nih.gov/pubmed/28411216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00291-17
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