A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs

Detection of DNA methylation in the genome has been possible for decades; however, the ability to deliberately and specifically manipulate local DNA methylation states in the genome has been extremely limited. Consequently, this has impeded our understanding of the direct effect of DNA methylation o...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Genome Res
Prif Awduron: Pflueger, Christian, Tan, Dennis, Swain, Tessa, Nguyen, Trung, Pflueger, Jahnvi, Nefzger, Christian, Polo, Jose M., Ford, Ethan, Lister, Ryan
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Cold Spring Harbor Laboratory Press 2018
Pynciau:
Method
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6071642/
https://ncbi.nlm.nih.gov/pubmed/29907613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.233049.117
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