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Construction of a Gene Knockdown System Based on Catalytically Inactive (“Dead”) Cas9 (dCas9) in Staphylococcus aureus

There has been an absence of an efficient method of gene knockdown in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The previously developed antisense RNA technology is mainly applied for forward genetic screening but is rather limited in specific gene knock...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Main Authors: Zhao, Changlong, Shu, Xueqin, Sun, Baolin
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5452804/
https://ncbi.nlm.nih.gov/pubmed/28411216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00291-17
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