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Construction of a Gene Knockdown System Based on Catalytically Inactive (“Dead”) Cas9 (dCas9) in Staphylococcus aureus

There has been an absence of an efficient method of gene knockdown in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The previously developed antisense RNA technology is mainly applied for forward genetic screening but is rather limited in specific gene knock...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Zhao, Changlong, Shu, Xueqin, Sun, Baolin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5452804/
https://ncbi.nlm.nih.gov/pubmed/28411216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00291-17
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