Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX)

LAGLIDADG homing endonucleases (LHEs) are a class of rare-cleaving nucleases that possess several unique attributes for genome engineering applications. An important approach for advancing LHE technology is the generation of a library of design ‘starting points’ through the discovery and characteriz...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Jacoby, Kyle, Lambert, Abigail R., Scharenberg, Andrew M.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2017
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5388424/
https://ncbi.nlm.nih.gov/pubmed/28180328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkw864
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