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Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA-Binding and Cleavage Properties

A fast, easy, and scalable method to assess the properties of site-specific nucleases is crucial to understanding their in cellulo behavior in genome engineering or population-level gene drive applications. Here we describe an analytical platform that enables high-throughput, semiquantitative interr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Baxter, Sarah K., Lambert, Abigail R., Scharenberg, Andrew M., Jarjour, Jordan
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4165528/
https://ncbi.nlm.nih.gov/pubmed/23423888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-293-3_4
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