Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases

LAGLIDADG homing endonucleases (LHEs) cleave 18–24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Volná, Petra, Jarjour, Jordan, Baxter, Sarah, Roffler, Steve R., Monnat, Raymond J., Stoddard, Barry L., Scharenberg, Andrew M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2007
Soggetti:
Nucleic Acid Enzymes
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1885675/
https://ncbi.nlm.nih.gov/pubmed/17426121
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm182
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi