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Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases
LAGLIDADG homing endonucleases (LHEs) cleave 18–24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1885675/ https://ncbi.nlm.nih.gov/pubmed/17426121 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm182 |
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