High-resolution profiling of homing endonuclease binding and catalytic specificity using yeast surface display

Experimental analysis and manipulation of protein–DNA interactions pose unique biophysical challenges arising from the structural and chemical homogeneity of DNA polymers. We report the use of yeast surface display for analytical and selection-based applications for the interaction between a LAGLIDA...

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Detalhes bibliográficos
Main Authors: Jarjour, Jordan, West-Foyle, Hoku, Certo, Michael T., Hubert, Christopher G., Doyle, Lindsey, Getz, Melissa M., Stoddard, Barry L., Scharenberg, Andrew M.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2009
Assuntos:
Nucleic Acid Enzymes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777416/
https://ncbi.nlm.nih.gov/pubmed/19740766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp726
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