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Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve endonucleas...
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2009
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2647301/ https://ncbi.nlm.nih.gov/pubmed/19103658 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn1007 |
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