Optimization of in vivo activity of a bifunctional homing endonuclease and maturase reverses evolutionary degradation

The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve endonucleas...

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Detalles Bibliográficos
Main Authors: Takeuchi, Ryo, Certo, Michael, Caprara, Mark G., Scharenberg, Andrew M., Stoddard, Barry L.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2009
Assuntos:
Nucleic Acid Enzymes
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2647301/
https://ncbi.nlm.nih.gov/pubmed/19103658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn1007
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