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Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering

Homing endonucleases (HEs) cleave long (∼20 bp) DNA target sites with high site specificity to catalyze the lateral transfer of parasitic DNA elements. In order to determine whether comprehensive computational design could be used as a general strategy to engineer new HE target site specificities, w...

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Detalhes bibliográficos
Main Authors: Ulge, Umut Y., Baker, David A., Monnat, Raymond J.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3105429/
https://ncbi.nlm.nih.gov/pubmed/21288879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr022
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