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Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease
We used a yeast one-hybrid assay to isolate and characterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cleavage-resistant I-PpoI target or ‘homing’ site DNA in vivo. Native I-PpoI recognizes and cleaves a semi-palindromic 15-bp target site with high speci...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
2007
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2034468/ https://ncbi.nlm.nih.gov/pubmed/17720708 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm624 |
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