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Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease

We used a yeast one-hybrid assay to isolate and characterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cleavage-resistant I-PpoI target or ‘homing’ site DNA in vivo. Native I-PpoI recognizes and cleaves a semi-palindromic 15-bp target site with high speci...

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Bibliografske podrobnosti
Main Authors: Eklund, Jennifer L., Ulge, Umut Y., Eastberg, Jennifer, Monnat, Raymond J.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2034468/
https://ncbi.nlm.nih.gov/pubmed/17720708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm624
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