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Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences

BACKGROUND: Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status of any gi...

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Bibliografske podrobnosti
izdano v:Clin Epigenetics
Main Authors: Candiloro, Ida L. M., Mikeska, Thomas, Dobrovic, Alexander
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2017
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5379501/
https://ncbi.nlm.nih.gov/pubmed/28392841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13148-017-0328-4
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