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Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays
BACKGROUND: The development of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that function under identical thermal cycling conditions. The use of a primer optimisation matrix to im...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2706253/ https://ncbi.nlm.nih.gov/pubmed/19549292 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-112 |
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