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Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
BACKGROUND: Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status of any gi...
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| Publié dans: | Clin Epigenetics |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5379501/ https://ncbi.nlm.nih.gov/pubmed/28392841 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13148-017-0328-4 |
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