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Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Multiplex bisulfite-PCR sequencing is a convenient and scalable method for the quantitative determination of the methylation state of target DNA regions. A challenge of this application is the presence of CpGs in the same region where primers are being placed. A common solution to the presence of Cp...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Lu, Jennifer, Ru, Kelin, Candiloro, Ida, Dobrovic, Alexander, Korbie, Darren, Trau, Matt
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5361148/
https://ncbi.nlm.nih.gov/pubmed/28327639
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep45096
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