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Quantitative detection of siRNA and single-stranded oligonucleotides: relationship between uptake and biological activity of siRNA
The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we describe a method for the detection of siRNA in extracts prepared from mammalian cells, which is based on liquid hybridization with a (32)P-labelle...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535697/ https://ncbi.nlm.nih.gov/pubmed/15576677 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh168 |
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