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Quantitative detection of siRNA and single-stranded oligonucleotides: relationship between uptake and biological activity of siRNA
The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we describe a method for the detection of siRNA in extracts prepared from mammalian cells, which is based on liquid hybridization with a (32)P-labelle...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535697/ https://ncbi.nlm.nih.gov/pubmed/15576677 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh168 |
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