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Quantitative detection of siRNA and single-stranded oligonucleotides: relationship between uptake and biological activity of siRNA

The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we describe a method for the detection of siRNA in extracts prepared from mammalian cells, which is based on liquid hybridization with a (32)P-labelle...

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Détails bibliographiques
Auteurs principaux: Overhoff, Marita, Wünsche, Winfried, Sczakiel, Georg
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2004
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC535697/
https://ncbi.nlm.nih.gov/pubmed/15576677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh168
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