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Predicting oligonucleotide-directed mutagenesis failures in protein engineering
Protein engineering uses oligonucleotide-directed mutagenesis to modify DNA sequences through a two-step process of hybridization and enzymatic synthesis. Inefficient reactions confound attempts to introduce mutations, especially for the construction of vast combinatorial protein libraries. This pap...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC535685/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15585664/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkh977 |
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