Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA...

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Dettagli Bibliografici
Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Dalbadie-McFarland, G, Cohen, L W, Riggs, A D, Morin, C, Itakura, K, Richards, J H
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1982
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Research Article
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347135/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6983070/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.21.6409
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