Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.

We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA...

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Detalhes bibliográficos
Main Authors: Dalbadie-McFarland, G, Cohen, L W, Riggs, A D, Morin, C, Itakura, K, Richards, J H
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC347135/
https://ncbi.nlm.nih.gov/pubmed/6983070
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