Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
We have used oligonucleotide-directed mutagenesis to make a specific change in the beta-lactamase (EC 3.5.2.6) (ampicillin resistance) gene of the plasmid pBR322. Evidence suggests that the active site for this enzyme may include a serine-threonine dyad (residues 70 and 71). By priming in vitro DNA...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1982
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347135/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6983070/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.21.6409 |
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