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A simple vector modification to facilitate oligonucleotide-directed mutagenesis.
We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low. The modification consists of the insertion...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1990
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC331175/ https://ncbi.nlm.nih.gov/pubmed/2115992 |
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