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A simple vector modification to facilitate oligonucleotide-directed mutagenesis.
We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low. The modification consists of the insertion...
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| Publicado no: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1990
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| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331175/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2115992/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.14.4175 |
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