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A simple vector modification to facilitate oligonucleotide-directed mutagenesis.

We describe a simple modification of commonly used single-stranded cloning vectors that permits the efficient recovery of mutant DNA molecules in oligonucleotide-directed mutagenesis experiments, even when the absolute efficiency of mutagenesis is very low. The modification consists of the insertion...

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Bibliografski detalji
Izdano u:Nucleic Acids Res
Glavni autori: Setzer, D R, Hmiel, R M, Liao, S Y
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1990
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC331175/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2115992/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.14.4175
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