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Fluorescent protein engineering by in vivo site-directed mutagenesis
In vivo site-directed mutagenesis by ssDNA recombineering is a facile method to change the color of fluorescent proteins without cloning. Two different starting alleles of GFP were targeted for mutagenesis: gfpmut3* residing in the E. coli genome and egfp carried by a bacterial/mammalian dual expres...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3415465/ https://ncbi.nlm.nih.gov/pubmed/22639380 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/iub.1041 |
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