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Predicting oligonucleotide-directed mutagenesis failures in protein engineering
Protein engineering uses oligonucleotide-directed mutagenesis to modify DNA sequences through a two-step process of hybridization and enzymatic synthesis. Inefficient reactions confound attempts to introduce mutations, especially for the construction of vast combinatorial protein libraries. This pap...
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| Main Authors: | , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
2004
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC535685/ https://ncbi.nlm.nih.gov/pubmed/15585664 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh977 |
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