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Concurrent measures of fusion and transduction efficiency of primary CD34+ cells with human immunodeficiency virus 1-based lentiviral vectors reveal different effects of transduction enhancers

Lentivirals vectors (LVs) are used for various gene transfer applications, notably for hematopoietic gene therapy, but methods are lacking to precisely evaluate parameters that control the efficiency of transduction in relation with the entry of vectors into target cells. We adapted a fluorescence r...

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Bibliographische Detailangaben
Veröffentlicht in:Hum Gene Ther Methods
Hauptverfasser: Ingrao, Dina, Majdoul, Saliha, Seye, Ababacar, Galy, Anne, Fenard, David
Format: Artigo
Sprache:Inglês
Veröffentlicht: Mary Ann Liebert, Inc 2014
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5299969/
https://ncbi.nlm.nih.gov/pubmed/24152219
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2013.090
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