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Kinetics of lentiviral vector transduction in human CD34(+) cells

Unlike cell lines, human hematopoietic stem cells (HSCs) are less efficiently transduced with HIV-1 vectors, potentially limiting this approach. To investigate which step (internalization, reverse transcription, nuclear transport, and integration) limits lentiviral transduction, we evaluated the kin...

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Détails bibliographiques
Publié dans:Exp Hematol
Auteurs principaux: Uchida, Naoya, Green, Rashidah, Ballantine, Josiah, Skala, Luke P., Hsieh, Matthew M., Tisdale, John F.
Format: Artigo
Langue:Inglês
Publié: 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4733401/
https://ncbi.nlm.nih.gov/pubmed/26499040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.exphem.2015.10.003
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