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Optimal conditions for lentiviral transduction of engrafting human CD34(+) cells

Cytokines are required for γ-retroviral transduction of human CD34(+) cells. However, cytokines may reduce engraftment of CD34(+) cells and may not be necessary for their lentiviral transduction. We sought to optimize transduction and engraftment of human CD34(+) cells using lentiviral vectors. Sing...

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Detalles Bibliográficos
Publicado en:Gene Ther
Main Authors: Uchida, N, Hsieh, MM, Hayakawa, J, Madison, C, Washington, KN, Tisdale, JF
Formato: Artigo
Idioma:Inglês
Publicado: 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6525965/
https://ncbi.nlm.nih.gov/pubmed/21544097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/gt.2011.63
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