MAR‐Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering

Untargeted plasmid integration into mammalian cell genomes remains a poorly understood and inefficient process. The formation of plasmid concatemers and their genomic integration has been ascribed either to non‐homologous end‐joining (NHEJ) or homologous recombination (HR) DNA repair pathways. Howev...

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Bibliographic Details
Published in:Biotechnol Bioeng
Main Authors: Kostyrko, Kaja, Neuenschwander, Samuel, Junier, Thomas, Regamey, Alexandre, Iseli, Christian, Schmid‐Siegert, Emanuel, Bosshard, Sandra, Majocchi, Stefano, Le Fourn, Valérie, Girod, Pierre‐Alain, Xenarios, Ioannis, Mermod, Nicolas
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2016
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5215416/
https://ncbi.nlm.nih.gov/pubmed/27575535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.26086
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