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Pronuclear Injection-based Targeted Transgenesis
Microinjection of DNA expression cassettes into fertilized zygotes has been a standard method for generating transgenic animal models. While efficient, the injected DNA integrates randomly into the genome, leading to potential problems such as disruption of endogenous genes or regulatory elements, v...
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| Yayımlandı: | Curr Protoc Hum Genet |
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| Asıl Yazarlar: | , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2016
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5123763/ https://ncbi.nlm.nih.gov/pubmed/27727435 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphg.23 |
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