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Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system
Targeted transgenic mouse models, where an exogenous gene is inserted into a specified genomic locus to achieve its stable and reliable expression, have been widely used in biomedical research. However, the available methodologies for targeted insertion of sequences require many laborious steps that...
Gardado en:
| Publicado en: | FEBS Open Bio |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4372609/ https://ncbi.nlm.nih.gov/pubmed/25834785 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2015.03.003 |
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