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Pronuclear Injection-based Targeted Transgenesis
Microinjection of DNA expression cassettes into fertilized zygotes has been a standard method for generating transgenic animal models. While efficient, the injected DNA integrates randomly into the genome, leading to potential problems such as disruption of endogenous genes or regulatory elements, v...
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| Publicado no: | Curr Protoc Hum Genet |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5123763/ https://ncbi.nlm.nih.gov/pubmed/27727435 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphg.23 |
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