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Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression
Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two d...
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| Main Authors: | , , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3001095/ https://ncbi.nlm.nih.gov/pubmed/20880997 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq860 |
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