Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

Mouse transgenesis has proven invaluable for analysis of gene function and generation of human disease models. We describe here the development of a pronuclear injection-based targeted transgenesis (PITT) system, involving site-specific integration in fertilized eggs. The system was applied to two d...

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מידע ביבליוגרפי
Main Authors: Ohtsuka, Masato, Ogiwara, Sanae, Miura, Hiromi, Mizutani, Akiko, Warita, Takayuki, Sato, Masahiro, Imai, Kenji, Hozumi, Katsuto, Sato, Takehito, Tanaka, Masafumi, Kimura, Minoru, Inoko, Hidetoshi
פורמט: Artigo
שפה:Inglês
יצא לאור: Oxford University Press 2010
נושאים:
Methods Online
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3001095/
https://ncbi.nlm.nih.gov/pubmed/20880997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq860
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