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Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System
CRISPR/Cas9 system has become a new versatile technology for genome engineering in various species. To achieve targeted modifications at the same site in both human and mice genomes by a CRISPR/Cas9 nuclease, we designed two target sites in conserved regions of vitamin D receptor (VDR) gene, which c...
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| 發表在: | PLoS One |
|---|---|
| Main Authors: | , , , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Public Library of Science
2016
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5042489/ https://ncbi.nlm.nih.gov/pubmed/27685656 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163551 |
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