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Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System

CRISPR/Cas9 system has become a new versatile technology for genome engineering in various species. To achieve targeted modifications at the same site in both human and mice genomes by a CRISPR/Cas9 nuclease, we designed two target sites in conserved regions of vitamin D receptor (VDR) gene, which c...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Zhang, Tao, Yin, Yajun, Liu, Huan, Du, Weili, Ren, Chonghua, Wang, Ling, Lu, Hongzhao, Zhang, Zhiying
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5042489/
https://ncbi.nlm.nih.gov/pubmed/27685656
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163551
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