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Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote
Microinjection of the CRISPR/Cas9 system in zygotes is an efficient and comparatively fast method to generate genetically modified mice. So far, only few knock-in mice have been generated using this approach, and because no systematic study has been performed, parameters controlling the efficacy of...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5314402/ https://ncbi.nlm.nih.gov/pubmed/28209967 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep42661 |
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