Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

Robust reproductive engineering techniques are required for the efficient and rapid production of genetically modified mice. We have reported the efficient production of genome-edited mice using reproductive engineering techniques, such as ultra-superovulation, in vitro fertilization (IVF) and vitri...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Biol Open
Main Authors: Nakagawa, Yoshiko, Sakuma, Tetsushi, Nishimichi, Norihisa, Yokosaki, Yasuyuki, Takeo, Toru, Nakagata, Naomi, Yamamoto, Takashi
Format: Artigo
Jezik:Inglês
Izdano: The Company of Biologists Ltd 2017
Teme:
Methods & Techniques
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5450330/
https://ncbi.nlm.nih.gov/pubmed/28396487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/bio.025122
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