Homology-driven genome editing in hematopoietic stem and progenitor cells using zinc finger nuclease mRNA and AAV6 donors

Genome editing with targeted nucleases and DNA donor templates homologous to the break site has proven challenging in human hematopoietic stem and progenitor cells (HSPCs), and particularly in the most primitive, long-term repopulating cell population. Here we report that combining electroporation o...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Wang, Jianbin, Exline, Colin M., DeClercq, Joshua J., Llewellyn, G. Nicholas, Hayward, Samuel B., Li, Patrick Wai-Lun, Shivak, David A., Surosky, Richard T., Gregory, Philip D., Holmes, Michael C., Cannon, Paula M
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4842001/
https://ncbi.nlm.nih.gov/pubmed/26551060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.3408
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